Prothonotary Warbler by Michelle Davis

Johnson Bayou Chenier


 

SITE DESCRIPTION

Chenier habitat

This site is a privately owned woodland close to the town of Johnson Bayou. The habitat is characterized by narrow strips of woodland dominated by hackberry (Celtis laevigata) and other tree species, including, in order of abundance, red mulberry (Morus rubra), live oak (Quercus virginiana), honeylocust (Gleditsia triacanthos), Japanese honeysuckle (Lonicera japonica) and tallowtree (Sapium sebiferum).  Understory species include yaupon (Ilex vomitoria), blackberry (Rubus L.sp.), greenbrier (Smilax tamnoides), poison ivy (Toxicodendron radicans), and cherokee rose (Rosa laevigata). Cattle grazing is common among the cheniers, leaving sparse understory and a simple vertical vegetative structure.


RESEARCH ACTIVITY

We've been working here since 1993. During the field season, approxiamately 25 "high" and "low" mist-nets are operated daily at the site, weather permitting.  We capture about 1,900 birds per season with an average effort of about 7000 net hours.  Over nine seasons of operation, 115 species of birds have been captured. 

Click here for a complete table of all the birds captured at the Johnson Bayou study site over the years.

We have also collected blood samples, feathers, fecal samples, and data on vegetation, arthropod abundance, and weather during some years.

The Banding Tent at Johnson Bayou

JENNIFER OWEN

Jen bleeding bird image

The main focus of Jen's research was to assess the immunocompetence of landbird migrants during spring passage. In particular, She was interested in whether birds trade-off immune function in response to the heightened demands of the migratory period. During migration a migrant is faced with many challenges en route, including variation in quality and availability of food, unfamiliarity with stopover habitat, predation risk, adverse weather, competition for resources, and novel parasite fauna. In anticipation of the energetic demands of migration, birds become hyperphagic, consuming food at an increased rate.  In other vertebrates, evidence exists that increased activity, food deprivation, malnutrition, and sleep deficit result in depressed immune function. Trans-Gulf migration provides the ideal opportunity in which to study the effect of prolonged, strenuous exercise on the avian immune system.

The focal species of her research were Swainson's Thrush (Catharus ustulatus), Wood Thrush (Hycochilia mustelina), Veery (Catharus fuscescens), Gray-cheeked Thrush (Catharus minimus), and Ovenbird (Seirus seirus).

 

For each focal species captured, blood were drawn via the brachial vein (see techniques)and collected in several 75mm heparanized capillary tubes.  A blood smear was made, fixed in 100% methyl alcohol and stained using a Giemsa/Wright combination stain. The blood was spun in a microhematocrit centrifuge. From the blood samples, she assayed hematocrit, white blood cell counts (absolute and differential), sedimentation rate, and IgG.

Several aviary experiments were conducted looking at nocturnal restlessness and immunocompetence.  Birds were held in individual cages within an indoor aviary. Each cage was equipped with two horizontal perches attached to microswitches which record hopping activity via a data logger. In addition to the hematological parameters mentioned above, we used a phytohemaggluttin (PHA) immune challenge test to assess the competence of the cell-mediated branch of the immune system.

The laboratory component of the project was located at the University of Southern Mississippi immunology lab under the direction of Dr. Bobby Middlebrooks and Dr. Rhonda Patterson. We have purified Swainson's Thrush IgG from eggs collected in both MI (courtesy of Robert J. Smith) and VT (courtesy of Kent McFarland, VINS).  The purified IgG was used to produce antiserum whichis being used in ELISAs to assess concentration levels of IgG of individual birds.

 

 

The University of Southern Mississippi. Last modified: 24 February, 2007 . Questions and Comments?
URL: http://www.usm.edu/mbrg/job.html
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